Affiliated Programs

 

Biotechnology Program Short Courses

Summer 2014

The Biotechnology Program offers the following short courses on various techniques used in biotechnology research. If you would like to receive training in any of the areas listed below, please register through UCD Conference and Event Services. Their link is provided below each of the courses and by selecting it, registration will be possible for that particular class.


FLOW CYTOMETRY COURSE


Haring Hall
Flow Cytometry equipment

Days, Dates and Times: July 14 - 18, 2014 Monday through Friday
Lead Instructors: Bridget McLaughlin, Technical Director, UC Davis Flow Cytometry Shared Resource
Facility: Genome Center and Tupper Hall

This comprehensive flow cytometry course is enhanced with an exciting lineup of interactive laboratory sessions using many of our UC Davis cytometers as well as new instrumentation and software to teach you to a variety of established and new approaches in cytometry. Special guest speakers will introduce new trends in flow cytometry and explain the physical and electronic concepts in light collection and signal processing. An optional “dinner with the experts” evening is planned and lunch sessions will provide a forum for questions and interaction with the instructors.

Course objectives:

  • Learn the fundamental concepts of how flow cytometers work
  • Discuss best practices in sample preparation for flow cytometry
  • Describe standard assays and techniques
  • Introduce new cutting edge technologies and applications for single cell analysis and genotyping
  • Demystify multicolor cell staining, compensation and analysis

Laboratory sessions include:

  • UC Davis MoFlo Cell Sorter: cell sorting theory and practical considerations 
  • UC Davis LSRII: Software setup for successful multicolor acquisition and analysis
  • Multispectral imaging flow cytometry using the Amnis FlowSight Imaging Cytometer
  • Cell proliferation with the Life Technologies Attune cytometer
  • Multiparameter Analysis of Cell Surface Markers, Cytokines, Transcription Factors, and Phosphoprotein Expression by Flow Cytometry: BD LSRII cytometer
  • FlowJo basic and advanced flow cytometry data analysis and panel design wizard
  • Detection of nascent mRNA in living cells using the Millipore easyCyte cytometer
  • Targeted gene expression in single cells using flow sorting and the Fluidigm BioMark

Special guest speakers:

  • Holden Maecker, Ph.D., Director, Stanford University Human Immune Monitoring Core
  • Jolene Bradford, Staff Scientist, Molecular Probes/Life Technologies Cytometry Reagents
  • Marty Bigos, Director, Stanford Shared Flow Cytometry Laboratory
  • Nikesh Kotecha, Ph.D., Cytobank software solutions for flow Cytometry

Register here

Enrollment is limited to 30 students (lecture/lab), 40 lecture only.

 

See photos of 2013's course herecamera

 

PROTEOMICS COURSE

Brett Phinney
proteomics lab

Days, Dates and Times: August 4 - 8, 2014
Lead Instructors: Brett Phinney, PhD, (UC Davis Proteomics Facility www.proteomics.ucdavis.edu , UC Davis Genome Center). Rich Eigenheer, Ph.D, Kerry Nugent (Michrom Bioresources), Barrett Smith & Rudy Alvarado
Facility: Genome Center and Science Lab Building (SLB)

This introductory proteome analysis workshop is designed to expose participants to fundamental technology platforms and current information in the field of proteomics. The course will cover bottom up quantitative and non-quantitative electrospray liquid chromatography tandem mass spectrometry (ESI-LC/MS/MS) and “multidimensional protein identification technology (Mudpit LC/LC/MS/MS)” based proteomics using complex mixtures of proteins.  Participants will perform in-gel digestions, phosphopeptide enrichment, identification, and database searching using open source software such as X! Tandem and OMSSA with MS/MS data generated during the course.  Lectures will cover: fundamentals of protein chemistry and mass spectrometry (MS), MS based protein identification, posttranslational modification of proteins, label free methods of protein quantitation, database searching using MS/MS data, and traditional tools of protein biochemistry.  Mass spectrometry and computer lab sessions will include hands on practical applications of the techniques, using state of the art instrumentation and software. Specific course topics may change due to sample and mass spectrometer availability

Enrollment is limited to 30 students (lecture/lab), 40 lecture only.

Register here

See photos of 2013's course herecamera

 

 

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